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Key Documents

GE29-0510-21

HisTrap™ High Performance

Cytiva 29-0510-21, pack of 1 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

Conditionnement

pack of 1 mL

Fabricant/nom de marque

Cytiva 29-0510-21

Disponibilité

not available in North America

Paramètres

flow rate
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

7 mm

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium)

Capacité

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.)
 ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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Description générale

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Application

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

Caractéristiques et avantages

  • High binding capacity, at least 40 mg/ml chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Informations légales

HisTrap is a trademark of Cytiva

Pictogrammes

Environment
GHS09

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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Protocoles

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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