GE17-3712-06

HisTrap^™ Excel

Cytiva 17-3712-06, pack of 5 × 5 mL

• Synonyme(s) : affinity resins, Ni Sepharose excel column, ion affinity chromatography column, IMAC column
• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• Forme: prepacked column
• Réticulation: cross-linked
• Conditionnement: pack of 5 × 5 mL
• Fabricant/nom de marque: Cytiva 17-3712-06
• Conditions de stockage: (20% Ethanol)
• Paramètres: 5 bar; 5 ml/min - 20 ml/min flow rate
• Technique(s): liquid chromatography (LC): suitable
• Taille du lit: 16 mm × 25 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 16 mm
• Matrice: highly cross-linked 6% agarose
• Groupe de la matrice active: Nickel
• Diamètre moyen: 90 μm
• cleaning: 2-14
• working range: 2-13
• Capacité: ≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)
• Technique de séparation: affinity

Description

Description générale

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

Application

Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose^™ excel medium resistant to chelating and reducing agents

Caractéristiques et avantages

• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
• Increase target protein yield and decrease degradation through reduced and simplified sample handling
• HisTrap excel columns allow direct purification of cell-free, unclarified samples
• HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
• HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.

The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.

Informations légales

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids