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GE17-5319-01

HisTrap™ Fast Flow

Cytiva 17-5319-01, pack of 5 × 1 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

Conditionnement

pack of 5 × 1 mL

Fabricant/nom de marque

Cytiva 17-5319-01

Paramètres

<20 mL/min flow rate
42 psi

Taille du lit

16 mm × 25 mm

Volume du lit

5 mL

D.I. de la colonne

16 mm

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

Capacité

~40 mg binding capacity(histidine-tagged protein)

Adéquation

suitable for bioprocess medium

Description générale

HisTrap™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Caractéristiques et avantages

  • High binding capacity, approx. 40 mg/mL medium.
  • Negligible leakage of Ni2+.
  • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogrammes

Environment
GHS09

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Cell disruption and membrane preparation

This page shows how to perform a cell disruption und prepare membranes with products from GE Healthcare.

Cell disruption and membrane preparation

This page shows how to perform a cell disruption und prepare membranes with products from GE Healthcare.

Affinity Chromatography in a Purification Strategy (CIPP)

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using GE Healthcare products.

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

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