GE17-0921-04
HiTrap® IMAC Fast Flow
Cytiva 17-0921-04, pack of 5 × 5 mL
Synonyme(s) :
HiTrap Chromatography Column, IMAC Fast Flow, IMAC Fast Flow Column
About This Item
Produits recommandés
Conditionnement
pack of 5 × 5 mL
Fabricant/nom de marque
Cytiva 17-0921-04
Paramètres
42 psi
Volume du lit
5 mL
D.I. de la colonne
16 mm
Matrice
6% cross-linked agarose
Taille des particules
45-165 μm
Diamètre moyen
90 μm
cleaning
2-14(Medium uncharged with metal ions.)
working range
3-12
Capacité
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Adéquation
suitable for bioprocess medium
Catégories apparentées
Description générale
Application
IMAC Sepharose™ 6 Fast FLow consists of 90 ?m beads of highly cross-linked Agarose to which a chelating group has been covalently coupled. This chelating group can be charged with suitable metal ions, specific for your target Protein of interest. The bead size of IMAC Sepharose™ 6 Fast FLow enables high flow properties, making the medium an excellent choice for scaling up purification at laboratory scale and purification of histidine-tagged Proteins at process scale. Low metal ion leakage means that the activity of the purified Protein is retained and the risk of precipitation reduced, which results in increased purity, activity, and yield of the target Protein. Leakage of metal ions into the eluted target Protein pool from IMAC Sepharose™ 6 Fast FLow is generally Low under normal conditions.
IMAC Sepharose™ 6 Fast FLow is highly stable and compatible with a wide range of common additives. This helps maintain biological activity and increase product yield, while at the same time greatly expanding the range of suitable operating conditions.
The medium is available prepacked in HiTrap® HiScreen™ and HiPrep™ column formats for convenient purification of histidine-tagged Proteins at laboratory scale. Initial purification using a 1 mL HiTrap® IMAC FF column can be scaled up further with the 5 mL column. Purification can be scaled up even more using 20 mL HiPrep™ IMAC FF 16/10 columns. Prepacked HiScreen™ IMAC FF with a 10 cm bed height is the format of choice for method optimization and process development.
Caractéristiques et avantages
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Optimal bead size for scaling up.
- High binding capacity.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Produit(s) apparenté(s)
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
Protocoles
This page shows how to purify histidine-tagged proteins using HiTrap IMAC HP and HiTrap IMAC FF from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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