GE17-0920-05

HiTrap^® IMAC High Performance

Cytiva 17-0920-05, pack of 5 × 5 mL

• Synonyme(s) : Metal affinity chromatography
• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• Conditionnement: pack of 5 × 5 mL
• Fabricant/nom de marque: Cytiva 17-0920-05
• Paramètres: 42 psi (H₂O at room temperature.)
• Taille du lit: 16 mm × 25 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 16 mm
• Matrice: highly cross-linked 6% agarose
• Taille moy. des particules: 34 μm
• cleaning: 2-14(Medium uncharged with metal ions.)
• working range: 3-12
• Capacité: ≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Description

Description générale

HiTrap^® IMAC HP is prepacked with IMAC Sepharose^™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

Application

IMAC Sepharose^™ HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.

Caractéristiques et avantages

• For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
• Conveniently charge with your metal of choice.
• Small bead size for high-performance purification.
• High binding capacity.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids