Pular para o conteúdo
Merck
  • Perilipin1 deficiency in whole body or bone marrow-derived cells attenuates lesions in atherosclerosis-prone mice.

Perilipin1 deficiency in whole body or bone marrow-derived cells attenuates lesions in atherosclerosis-prone mice.

PloS one (2015-04-10)
Xiaojing Zhao, Mingming Gao, Jinhan He, Liangqiang Zou, Ying Lyu, Ling Zhang, Bin Geng, George Liu, Guoheng Xu
RESUMO

The objective of this study is to determine the role of perilipin 1 (Plin1) in whole body or bone marrow-derived cells on atherogenesis. Accumulated evidence have indicated the role of Plin1 in atherosclerosis, however, these findings are controversial. In this study, we showed that Plin1 was assembled and colocalized with CD68 in macrophages in atherosclerotic plaques of ApoE-/- mice. We further found 39% reduction of plaque size in the aortic roots of Plin1 and ApoE double knockout (Plin1-/-ApoE-/-) females compared with ApoE-/- female littermates. In order to verify whether this reduction was macrophage-specific, the bone marrow cells from wild-type or Plin1 deficient mice (Plin1-/-) were transplanted into LDL receptor deficient mice (LDLR-/-). Mice receiving Plin1-/- bone marrow cells showed also 49% reduction in aortic atherosclerotic lesions compared with LDLR-/- mice received wild-type bone marrow cells. In vitro experiments showed that Plin1-/- macrophages had decreased protein expression of CD36 translocase and an enhanced cholesterol ester hydrolysis upon aggregated-LDL loading, with unaltered expression of many other regulators of cholesterol metabolism, such as cellular lipases, and Plin2 and 3. Given the fundamental role of Plin1 in protecting LD lipids from lipase hydrolysis, it is reasonably speculated that the assembly of Plin1 in microphages might function to reduce lipolysis and hence increase lipid retention in ApoE-/- plaques, but this pro-atherosclerotic property would be abrogated on inactivation of Plin1. Plin1 deficiency in bone marrow-derived cells may be responsible for reduced atherosclerotic lesions in the mice.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Colesterol, Sigma Grade, ≥99%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
1,3-dimetil-2-imidazolidinona, ≥99.0% (GC)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Colesterol, powder, BioReagent, suitable for cell culture, ≥99%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
1,3-dimetil-2-imidazolidinona, absolute, over molecular sieve (H2O ≤0.04%), ≥99.5% (GC)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Colesterol, from sheep wool, ≥92.5% (GC), powder
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
SAFC
Colesterol, SyntheChol®
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
1,3-dimetil-2-imidazolidinona, reagent grade
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Supelco
Cholesterol solution, certified reference material, 10 mg/mL in chloroform
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Supelco
Colesterol, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Colesterol, from lanolin, ≥99.0% (GC)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
SAFC
Colesterol, from sheep wool, Controlled origin, meets USP/NF testing specifications
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Colesterol, tested according to Ph. Eur.
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade