814449

TSKgel^® Boronate-5PW (10 µm) HPLC Columns

L x I.D. 5 cm × 5 mm, m-aminophenyl boronate phase

• Sinônimo(s): TSKgel^® Boronate-5PW HPLC Column
• Código UNSPSC: 41115711

Propriedades

• product name: TSKgel^® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
• Materiais: glass
• linha de produto: TSKgel^®
• classe(s) química(is) do analito: proteins
• fabricante/nome comercial: Tosoh 14449
• Parâmetros: ≤20% organic solvent; ≤3 M salt concentration; >0 °C temperature; 1.2 mL/min flow rate; 30 bar max. pressure
• técnica(s): HPLC: suitable
• C × D.I.: 5 cm × 5 mm
• matriz: hydroxylated methacrylic polymer
• Grupo ativo da matriz: m-aminophenyl boronate phase
• tamanho de partícula: 10 μm
• pH operacional: 2-9
• capacidade: 40 μmol/mL, resin adsorption capacity (sorbitol)
• técnica de separação: affinity
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

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Informações legais

TSKgel is a registered trademark of Tosoh Corporation