567507-U

Discovery^® HS F5 (3 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Sinônimo(s): Discovery Pentafluorophenyl PFP HPLC Column
• Código UNSPSC: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Propriedades

• Nome do produto: Discovery^® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
• Materiais: stainless steel column
• Nível de qualidade: 100
• Agency: suitable for USP L43
• linha de produto: Discovery^®
• Características: endcapped
• fabricante/nome comercial: Discovery^®
• embalagem: 1 ea of
• Extensão da rotulagem: 12% Carbon loading
• Parâmetros: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• técnica(s): HPLC: suitable; LC/MS: suitable
• C × D.I.: 15 cm × 4.6 mm
• área da superfície: 300 m²/g
• cobertura de superfície: 4 μmol/m²
• Impurezas: <10 ppm metals
• Matriz: silica gel, high purity, spherical particle platform; fully porous particle
• Grupo ativo da matriz: PFP (pentafluorophenyl) phase
• tamanho de partícula: 3 μm
• tamanho de poro: 120 Å
• Faixa de pH operacional: 2-8
• aplicação(ões): food and beverages
• técnica de separação: reversed phase

Descrição

Descrição geral

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Aplicação

• Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.: This study uses the Discovery^® HS F5 HPLC Column to identify and quantify the main contaminant in primaquine diphosphate, crucial for ensuring the quality and safety of this antimalarial drug (Brondz et al., 2007).
• HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant.: Demonstrates the application of the Discovery^® HS F5 HPLC Column in the biologically relevant chemical analysis of Indian medicinal plants, contributing to the standardization of herbal medicine (Pullela et al., 2006).
• Disposition study of a new potential antineoplastic agent dimefluron in rats using high-performance liquid chromatography with ultraviolet and mass spectrometric detection.: Utilizes the Discovery^® HS F5 HPLC Column to study the pharmacokinetics and metabolism of a novel anticancer agent, aiding in the development of effective cancer therapies (Císar et al., 2005).
• A simplified analytical method for a phenotyping cocktail of major CYP450 biotransformation routes.: This research employs the Discovery^® HS F5 HPLC Column to simplify the analysis of a phenotyping cocktail, enhancing the assessment of drug metabolism and facilitating personalized medicine approaches (Jerdi et al., 2004).

Características e benefícios

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

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Informações legais

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany