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Key Documents

53829-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Sinônimo(s):

Coluna de HPLC Core-shell (SPP) fundida Core C18

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Coluna de HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Materiais

stainless steel column

Nível de qualidade

Agency

suitable for USP L1

linha de produto

Ascentis®

Características

endcapped

fabricante/nome comercial

Ascentis®

embalagem

1 ea of

Parâmetros

60 °C temp. range
600 bar max. pressure (9000 psi)

técnica(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

C × D.I.

15 cm × 4.6 mm

área da superfície

135 m2/g

Impurezas

<5 ppm metals

matriz

Fused-Core particle platform
superficially porous particle

Grupo ativo da matriz

C18 (octadecyl) phase

tamanho de partícula

2.7 μm

tamanho de poro

90 Å

pH operacional

2-9

aplicação(ões)

food and beverages

técnica de separação

reversed phase

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Descrição geral

Colunas de HPLC Ascentis Express, através do uso tecnologia de partículas Fused-Core®, podem fornecer a alta velocidade e as altas taxas de eficiência de partículas com menos de 2 μm e, ao mesmo tempo, manter contrapressões menores. A combinação de alta eficiência e baixa contrapressão beneficia usuários de UPLC® (ou outro sistema de pressão ultra alta), além de usuários de HPLC convencionais.
Visite a página inicial do Ascentis Express para obter mais informações sobre essa nova tecnologia de colunas.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within

Artigos

Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of columns are specified. Therefore, the USP methods are under continuous revision to improve existing procedures or to allow the user to obtain better results.

Apixaban is sold under the tradename Eliquis and at present no pharmacopeial monographs are available for Axpixaban using either an assay and/or organic impurity profiling method. A newly developed and validated HPLC-UV method using a column with C18 solid core particles is demonstrated.

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

Sigma-Aldrich presents an article on serum lipidomics profiling using an Ascentis Express Fused-Core C18 column.

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Protocolos

This paper illustrates the setting up of a Lopinavir assay following European pharmacopoeia guidelines (10.2), and using a shorter column packed with smaller particles. The described assay saves valuable time and improves separation efficiency, which typically translates to better method performance.

This method is particularly useful in research into the role of individual bile acids as signaling molecules; suitable for clinical laboratories to investigate potential mechanisms linked to gut hormone profiles and glycemic control.

HPLC Analysis of Benzene and Deuterated Benzene on Ascentis® Express C18

Conteúdo relacionado

Ginsenosides Separation in Ginseng. The HPLC method was first optimized using a ginsenoside standard mixture, and was then applied to a sample of American Ginseng root.

Bile Acids (BA) are synthesized in the liver and play important roles in cholesterol homeostasis, absorption of vitamins and lipids, and various key metabolic processes.

Chromatograms

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