ZRB2454

Anti-Ly-6C1 Antibody, clone 2H5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinônimo(s): Anti-TGF β receptor 1 Antibody, clone 4H18 ZooMAb^® Rabbit Monoclonal, Ly-6C.1, Lymphocyte antigen 6C
• Código UNSPSC: 12352203
• NACRES: NA.43
• conjugado: unconjugated
• application: FACS; ICC; IHC; WB; affinity binding assay
• clone: 2H5, recombinant monoclonal
• reatividade de espécies: mouse
• técnica(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 2H5, recombinant monoclonal
• descrição: recombinant, expressed in HEK 293 cells
• linha de produto: ZooMAb^® learn more
• Formulário: lyophilized
• peso molecular: calculated mol wt 14.19 kDa
• purificado por: using Protein A
• reatividade de espécies: mouse
• embalagem: antibody small pack of 25
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG
• sequência de epítopo: Internal
• nº de adesão de ID de proteína: NP_001238984.1
• nº de adesão UniProt: P0CW02
• categoria alternativa mais ecológica: Aligned
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: mouse ... Ly6c1(17067)

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone 2H5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Lymphocyte antigen 6C (Ly-6C1). It targets an epitope within 15 amino acids from the internal region.

Imunogênio

Clone 2H5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Lymphocyte antigen 6C (Ly-6C1). It targets an epitope within 15 amino acids from the internal region.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Ly-6C1 in C2C12 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ly-6C1 in NIH3T3 cell lysates.

Flow Cytometry Analysis: 1 µg from a representative lot detected Ly-6C1 in one million C57BL6 Mouse splenocytes.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ly-6C1 in C2C12 cells.

Affinity Binding Assay: A representative lot of this antibody bound Ly-6C1 peptide with a KD of 5.2 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ly-6C1 in Mouse spleen tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Lymphocyte antigen 6C (UniProt: P0CW02; also known as Ly-6C, Ly-6C.1) is encoded by the Ly6c1 gene (Gene ID: 17067) in murine species. Ly-6 molecules are encoded by multiple tightly linked genes located on mouse chromosome 15 and have conserved genomic organization. They are expressed on fully differentiated cells in specific compartments of peripheral lymphoid tissues. Ly-6 proteins are widely used as differentiation markers on hematopoietic cells. LY-6C is a GPI-anchor protein that is synthesized with a signal peptide (aa 1-26) and a propeptide (aa 110-131) that are subsequently cleaved off to produce the mature form (aa 27-109). It contains one UPAR/Ly6 domain that is localized in amino acids 27-115. Its expression is observed in monocytes/macrophages, endothelial cells, bone marrow cells, neutrophils, and some subsets of T cells. Its expression is considered as a superior marker to identify monocyte and macrophage subpopulations. Ly6C+ macrophages are believed to be derived from

forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstituição

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable