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Key Documents

ZRB1366

Sigma-Aldrich

Anti-Erythropoietin Antibody, clone 6E5, ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinônimo(s):

EPO, Epoetin

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

fonte biológica

rabbit (recombinant)

Nível de qualidade

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

6E5, monoclonal
recombinant monoclonal

linha de produto

ZooMAb® learn more

forma

lyophilized

peso molecular

calculated mol wt 21.31 kDa
observed mol wt ~20 kDa

reatividade de espécies

rat, human, mouse

embalagem

antibody small pack of 25 μL

características do produto alternativo mais ecológico

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validação aprimorada

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

técnica(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotipo

IgG

nº de adesão UniProt

categoria alternativa mais ecológica

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... EPO(2056)

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidade

Clone 6E5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Erythropoietin.

Imunogênio

His-tagged full-length mature recombinant human Erythropoietin (without the signal peptide).

Aplicação

Anti-Erythropoietin, clone 6E5 ZooMAb, Cat. No. ZRB1366, a recombinant Rabbit Monoclonal Antibody that targets Erythropoietin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Erythropoietin in HUVEC and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Erythropoietin in human kidney and rat kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Erythropoietin (UniProt: P01588; also known as Epoetin, EPO) is encoded the EPO gene (Gene ID: 2056) in human. EPO is an acidic glycoprotein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off in the endoplasmic reticulum and the mature protein undergoes glycosylation in the Golgi prior to its release. EPO is mainly produced by peritubular fibroblasts in the renal cortex and to some extent by the liver in adult mammals. In fetal and neonatal mammals, it produced in the liver. EPO is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. It is induced by ischemic preconditioning by activation of (HIF)-1alpha. It is reported to contribute to angiogenesis in response to ischemia through upregulation of VEGF/VEGFR. It binds to EPO receptor that leads to receptor dimerization and JAK2 activation that results in activation of specific downstream effectors, including STAT1 and STAT3. Endogenous EPO consists of three N-linked polysaccharide groups and one O-linked group. Although its glycosylation slightly reduces its biological activity but is shown to be essential for ensuring its prolonged circulation in the plasma. The N-glycans in its structure protect it from the action of proteases and in the modulation of its receptor binding affinity. Mutations in EPO gene can cause microvascular complications in type II diabetic subjects and erythrocytosis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bunn, HF (2013). Cold Spring Harb. Perspect. Med. 3(3); a011619).

forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstituição

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1


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