W0138
Western Blocker™ Solution
for HRP detection systems
Sinônimo(s):
Western blot blocking solution, blocking buffer
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About This Item
Código UNSPSC:
41105329
NACRES:
NA.25
Produtos recomendados
![Western Blocking Reagent, Solution solution, sufficient for 10 blots (11921673001 [100 cm2]), sufficient for 60 blots (11921681001 [100 cm2])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
Descrição geral
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Aplicação
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Características e benefícios
- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Informações legais
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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