T9784

Tricina

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

• Sinônimo(s): N-[Tris(hidroximetil)metil]glicina
• Fórmula linear: (HOCH₂)₃CNHCH₂CO₂H
• Número CAS: 5704-04-1
• Peso molecular: 179.17
• Beilstein: 1937804
• Número CE: 227-193-6
• Número MDL: MFCD00004277
• Código UNSPSC: 12161700
• ID de substância PubChem: 24900603
• NACRES: NA.25

Propriedades

• linha de produto: BioXtra
• Nível de qualidade: 400
• Ensaio: ≥99% (titration)
• forma: powder
• Impurezas: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• resíduo de ignição: ≤0.1%
• pH: 4.0-6.0 (1 M in H₂O)
• faixa de pH útil: 7.4-8.8
• pKa (25 °C): 8.1
• solubilidade: H₂O: 1 M, clear, colorless
• traços de ânion: chloride (Cl^-): ≤0.1%; sulfate (SO₄^2-): ≤0.05%
• traços de cátion: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• absorção: ≤0.05 at 280 in H₂O at 1 M; ≤0.1 at 260 in H₂O at 1 M
• aplicação(ões): diagnostic assay manufacturing
• cadeia de caracteres SMILES: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• chave InChI: SEQKRHFRPICQDD-UHFFFAOYSA-N

Descrição

Descrição geral

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Aplicação

Tricine has been used:

• in a polymerase chain reaction (PCR) buffer used in the single-cell collection
• as a component of lactotroph feeding medium for culturing lactotrophs
• to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer

Componente de tampão para separação de peptídeos de baixo peso molecular.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)