T7539
Tumor Necrosis Factor-α from mouse
≥95%, recombinant, expressed in E. coli, powder, suitable for cell culture
Sinônimo(s):
mTNF-α, TNF-α
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
Nome do produto
Tumor Necrosis Factor-α from mouse, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
fonte biológica
mouse
Nível de qualidade
recombinante
expressed in E. coli
Ensaio
≥95%
Formulário
powder
potência
0.01-0.5 ng/mL EC50
peso molecular
17 kDa
embalagem
pkg of 10 μg
pkg of 50 μg
condição de armazenamento
avoid repeated freeze/thaw cycles
técnica(s)
cell culture | mammalian: suitable
Impurezas
≤1 EU/μg protein endotoxin
cor
white
solubilidade
water: soluble
nº de adesão UniProt
temperatura de armazenamento
−20°C
Informações sobre genes
mouse ... Tnf(21926)
Aplicação
Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
- to activate MS1 endothelium cells
- to induce lipolysis in adipocytes
- to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation
Ações bioquímicas/fisiológicas
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.
Tumor necrosis factor-α, also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.
Definição da unidade
One unit is the amount required to induce half-maximal cytolysis of L929 cells.
forma física
Lyophilized from 10 mM sodium phosphate, pH 7.5
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Skin Irrit. 2
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-induced lipolysis in adipocytes
Yang X, et al.
The Journal of Biological Chemistry, 286(47), 40477-40485 (2011)
SDF-1--CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1--SLIT2 interactions in mice
Glawe JD, et al.
Diabetologia, 56(10), 2222-2230 (2013)
Glucocorticoid-independent repression of tumor necrosis factor (TNF) $\alpha$-stimulated interleukin (IL)-6 expression by the glucocorticoid receptor a potential mechanism for protection against an excessive inflammatory response
Verhoog NJD, et al.
The Journal of Biological Chemistry, 286(22), 19297-19310 (2011)
Jing Deng et al.
Molecular and cellular biology, 24(23), 10161-10168 (2004-11-16)
Numerous stressful conditions activate kinases that phosphorylate the alpha subunit of translation initiation factor 2 (eIF2alpha), thus attenuating mRNA translation and activating a gene expression program known as the integrated stress response. It has been noted that conditions associated with
Matteo Cornaglia et al.
Analytical chemistry, 86(16), 8213-8223 (2014-07-30)
We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive stochastic reorientations in
Artigos
Lipid Induced Insulin Resistance
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica