T3809

TRI Reagent^®

BD, For processing whole blood, plasma, or serum.

• Sinônimo(s): TRI Reagent^® Reagente de isolamento de RNA
• Número MDL: MFCD00213058
• Código UNSPSC: 12352200
• NACRES: NA.52

Propriedades

• Nível de qualidade: 200
• uso: 0.75 mL sufficient for 0.25 mL blood derivatives

Descrição

Descrição geral

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.

TRI Reagent^® is a monophasic solution of phenol and guanidinium isothiocyanate.

Aplicação

TRI Reagent^® has been used for the isolation of RNA.

The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.

O reagente TRI é uma versão aprimorada do reagente de isolamento de RNA total em uma etapa desenvolvido por Chomczynski. O método de isolamento de RNA baseado neste reagente é amplamente utilizado e aprovado para aplicações com RNA. Ele é ideal para o isolamento rápido, econômico e eficiente de RNA total ou isolamento simultâneo de RNA, DNA e proteínas de amostras de origem humana, vegetal, de levedura, bacteriana e viral.

Características e benefícios

• Isolamento de RNA fácil de escalonar.
• Funciona com várias fontes: humana, vegetal, de leveduras, bacteriana ou viral.
• Melhor rendimento que os métodos tradicionais de tiocianato de gianidina/cloreto de césio.

Informações legais

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Informações de segurança

• Pictogramas: GHS06,GHS08,GHS05,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H301 + H311 + H331,H314,H341,H373,H411
• Declarações de precaução: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• Órgãos-alvo: Nervous system,Kidney,Liver,Skin
• Perigos de suplementos: EUH032
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 174.2 °F - closed cup
• Ponto de fulgor (°C): 79 °C - closed cup