Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

View All Documentation

T3405

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

chromogenic, tablet

Sinônimo(s):

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Fórmula empírica (Notação de Hill):
C16H20N2 · 2HCl
Número CAS:
64285-73-0
Peso molecular:
313.27
Número CE:
264-769-6
Número MDL:
MFCD00012961
Código UNSPSC:
12352204
ID de substância PubChem:
24900140
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

forma

tablet

pf

≥300 °C

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

chave InChI

NYNRGZULARUZCC-UHFFFAOYSA-N

Procurando produtos similares? Visita Guia de comparação de produtos

Aplicação

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
  • anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
  • anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
  • in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Yan-Qin Huang et al.
Analytical and bioanalytical chemistry, 410(28), 7385-7394 (2018-09-15)
We certify that protamine-gold nanoclusters (PRT-AuNCs) synthesized by one-pot method exhibit peroxidase-like activity. The catalytic activity of PRT-AuNCs followed typical Michaelis-Menten kinetics and exhibited higher affinity to 3,3',5,5'-tetramethylbenzidine (TMB) as the substrate compared to that of natural horseradish peroxidase. Meanwhile
Beth C Holbrook et al.
Virology, 476, 124-133 (2014-12-30)
Respiratory infection of young infants results in increased morbidity and mortality compared to infection of adults. In spite of the significance of this health issue, our understanding of the immune response elicited in infants especially in the respiratory tract is
Targeting phospho-Ser422 by active Tau Immunotherapy in the THYTau22 mouse model: a suitable therapeutic approach
Troquier L, et al.
Current Alzheimer Research, 9(4), 397-405 (2012)
Darcy S O Mora et al.
Fish & shellfish immunology, 71, 255-263 (2017-09-04)
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated
Thiru Vanniasinkam et al.
Methods in molecular biology (Clifton, N.J.), 1785, 121-128 (2018-05-02)
This chapter describes a strategy for mapping linear B-cell epitopes of proteins using synthetic biotinylated peptides in an ELISA.A set of overlapping peptides were designed based upon a known amino acid sequence of the target protein, VapA (Virulence-associated Protein A)
Exibir todos os documentos relacionados

Artigos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica