SRP3255

TPO from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinônimo(s): MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand
• Código UNSPSC: 12352202
• NACRES: NA.75

Propriedades

• fonte biológica: rat
• recombinante: expressed in E. coli
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formulário: lyophilized
• potência: ≤0.2 ng/mL ED₅₀
• peso molecular: 18.7 kDa
• embalagem: pkg of 10 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: white
• nº de adesão UniProt: P49745
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: rat ... THPO(81811)

Descrição

Descrição geral

Thrombopoietin (TPO) also known as c-Mpl ligand is an acidic glycoprotein expressed mainly in the liver, kidney, and bone marrow. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Aplicação

Thrombopoietin (TPO) from rat has been used in experimental systems to determine the effect of TPO in recovery from thrombocytopenia.

Ações bioquímicas/fisiológicas

Thrombopoietin (TPO) acts as a key physiologic regulator of megakaryocyte and platelet production in vivo. Mutation of TPO or its receptor might lead to thrombocytopenia and megakaryocytopoiesis. In addition, TPO also influence the action of erythroid and erythromegakaryocytic progenitors in mouse embryonic yolk sac. TPO binds to c-mpl receptor and regulates circulating platelet level.

Sequência

SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

forma física

Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable