SRP3196

EGF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinônimo(s): Epidermal Growth Factor, URG, Urogastrone
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: mouse
• recombinante: expressed in E. coli
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• forma: lyophilized
• potência: 0.1 ng/mL
• peso molecular: 6.0 kDa
• embalagem: pkg of 500 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: off-white to yellow
• nº de adesão UniProt: P01132
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: mouse ... EGF(13645)

Descrição

Descrição geral

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.

Aplicação

EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.

Ações bioquímicas/fisiológicas

EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.

Sequência

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

forma física

Lyophilized from 10 mM Sodium Phosphate, pH 8.2.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable