SRP3238

EGF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinônimo(s): Epidermal Growth Factor, URG, Urogastrone
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: rat
• recombinante: expressed in E. coli
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• forma: lyophilized
• potência: ≤0.1 ng/mL
• peso molecular: 6.2 kDa
• embalagem: pkg of 100 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: white to off-white
• nº de adesão UniProt: P07522
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: rat ... EGF(25313)

Descrição

Descrição geral

Epidermal growth factor (EGF) is a potent growth factor and a cytoprotective peptide. Recombinant rat EGF is a 6.2kDa globular protein containing 54 amino acid residues, including 3 intramolecular disulfide-bonds.

Aplicação

Epidermal growth factor (EGF) is used in hepatocyte culture to determine the optimal condition for survival and function of rat hepatocytes.

Ações bioquímicas/fisiológicas

Epidermal growth factor (EGF) stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, inflammation and is involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α (transforming growth factor-α) and VGF (vaccinia virus growth factor).

Sequência

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

forma física

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable