SRP0291
Cathepsin L Active human
recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)
Sinônimo(s):
CATL, Major excreted protein (MEP)
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About This Item
Código UNSPSC:
12352204
NACRES:
NA.54
Produtos recomendados
recombinante
expressed in FreeStyle™ 293-F cells
Ensaio
≥90% (SDS-PAGE)
forma
aqueous solution
peso molecular
36 kDa
nº de adesão NCBI
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... CTSL(1514)
Descrição geral
Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.
Aplicação
Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used in a study to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases. Active human cathepsin L has also been used in a study to investigate the role of cathepsin B and L activity in the serum during the human aging process.
Definição da unidade
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C
forma física
Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.
Nota de preparo
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Informações legais
FreeStyle is a trademark of Invitrogen Corp.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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Ricardo A Orbegozo-Medina et al.
PloS one, 14(2), e0211035-e0211035 (2019-02-02)
Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host
Jana Ilgová et al.
Molecular and biochemical parasitology, 235, 111248-111248 (2019-12-25)
The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably
Elda A Treviño et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(2), 490-502 (2018-11-21)
The etiology of joint tissue degeneration following rotator cuff tear remains unclear. Thus, the purpose of this study was to understand the timeline of protease activity in the soft tissues of the shoulder (tendon, muscle, and cartilage) that may lead
Aleksandra Wyczałkowska-Tomasik et al.
Archives of gerontology and geriatrics, 55(3), 735-738 (2012-06-15)
Cathepsin B and L are active in several physiological processes that occur in the human body. Their synthesis and activity is detectable in several tissues and processes, including skin hyperkeratosis, insemination, and embryonic development. Proteolytic enzymes are very important cellular
Ana Bela Santana et al.
Bioorganic & medicinal chemistry letters, 22(12), 3993-3997 (2012-05-19)
The synthesis, inhibitory activity and mode of action of oxazolidine-2,4-diones against porcine pancreatic elastase, here used as a model for human neutrophil elastase, are reported. The nature of N-substitution at the oxazolidine-2,4-dione scaffold has large effect on the inhibitory potency
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