SRP0216
SHP-1 Active human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Sinônimo(s):
HCP, Hematopoietic cell phosphatase, PTP-1C, PTPN6, SHP-1L
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
Produtos recomendados
fonte biológica
human
recombinante
expressed in E. coli
Ensaio
≥90% (SDS-PAGE)
forma
aqueous solution
peso molecular
95 kDa
embalagem
pkg of 20 μg
condição de armazenamento
avoid repeated freeze/thaw cycles
concentração
1 mg/mL
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... PTPN6(5777)
Descrição geral
Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, MW = 95 kDa, expressed in an E. coli expression system.
The Src homology region 2 domain-containing phosphatase-1 (SHP1)/protein tyrosine phosphatase, non-receptor type 6 (PTPN6) is a 68 kDa protein present in the cytoplasm. It is mapped to human chromosome 12p13.
Aplicação
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Ações bioquímicas/fisiológicas
In mammary epithelial cells, protein tyrosine phosphatase, non-receptor type 6 (PTPN6) controls EGFR (epidermal growth factor receptor) signaling. It participates in the JAK/STAT (janus kinase/signal transducers and activators of transcription) signaling pathway. PTPN6 regulates cytokine receptors like interleukin- 3 (IL-3) and erythropoietin.
Definição da unidade
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
forma física
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Nota de preparo
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Priya Saju et al.
Nature microbiology, 1, 16026-16026 (2016-08-31)
Most if not all gastric cancers are associated with chronic infection of the stomach mucosa with Helicobacter pylori cagA-positive strains(1-4). Approximately 10% of gastric cancers also harbour Epstein-Barr virus (EBV) in the cancer cells(5,6). Following delivery into gastric epithelial cells
Shp1 positively regulates EGFR signaling by controlling EGFR protein expression in mammary epithelial cells
Yuan T, et al.
Biochemical and Biophysical Research Communications, 488(3), 439-444 (2017)
Promoter methylation attenuates SHP1 expression and function in patients with primary central nervous system lymphoma
Liu J, et al.
Oncology Reports, 37(2), 887-894 (2017)
Wei Luo et al.
Nature immunology, 20(6), 736-746 (2019-04-24)
B cell antigen receptor (BCR) and CD40 signaling are rewired in germinal center (GC) B cells (GCBCs) to optimize selection for high-affinity B cells. In GCBC, BCR signals are constrained, but the mechanisms are not well understood. Here we describe
A Beghini et al.
Human molecular genetics, 9(15), 2297-2304 (2000-09-26)
The SH2 domain-containing tyrosine phosphatase PTPN6 (SHP-1, PTP1C, HCP) is a 68 kDa cytoplasmic protein primarily expressed in hematopoietic cell development, proliferation and receptor-mediated mitogenic signaling pathways. By means of direct dephosphorylation, it down-regulates a broad spectrum of growth-promoting receptors
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