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SEQR

Sigma-Aldrich

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Sinônimo(s):

WGA kit, Whole transcriptome amplification

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About This Item

Código UNSPSC:
41121800
NACRES:
NA.55

técnica(s)

whole genome amplification: suitable

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Aplicação

SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Características e benefícios

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Outras notas

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Informações legais

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

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Código de classe de armazenamento

11 - Combustible Solids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Cecilia Lindestam Arlehamn et al.
Journal of immunology (Baltimore, Md. : 1950), 193(6), 2931-2940 (2014-08-06)
In latent tuberculosis infection (LTBI) spread of the bacteria is contained by a persistent immune response, which includes CD4(+) T cells as important contributors. In this study we show that TB-specific CD4(+) T cells have a characteristic chemokine expression signature

Protocolos

SEQR - SeqPlex RNA Amplification Kit Protocol

SEQR - SeqPlex RNA Amplification Kit Protocol

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