SAB4500362

Anti-Collagen I antibody produced in rabbit

affinity isolated antibody

• Sinônimo(s): α 2 type i collagen, co1a2, col1a2, collagen, type i
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen 129 kDa
• reatividade de espécies: mouse, human, rat
• concentração: ~1 mg/mL
• técnica(s): ELISA: 1:5000; immunofluorescence: 1:100-1:500; immunohistochemistry: 1:50-1:100
• nº de adesão NCBI: P08123
• nº de adesão UniProt: P08123
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... COL1A2(1278)

Descrição

Descrição geral

Collagen I is encoded by the gene COL1A2 (collagen type I alpha 2). The gene is located on human chromosome 7q21.3. COL1A2 is a fibril forming collagen, found in bone, dermis, tendon, ligaments and cornea.

Collagen is the major structural component of extracellular matrices found in connective tissues and internal organs, but is most prevalent in the dermis, tendons, and bones. It is classified into a number of structurally and genetically distinct types. Type I collagen is a heterodimer composed of two α1(I) chains and one α2(I) chain that spontaneously forms a triple helix scaffold at neutral pH and 37 °C. Collagen type I is an excellent substrate for the culture of hepatocytes, fibroblasts, spinal ganglion, muscle cells, Schwann cells, embryonic lung cells, epithelial cells, and a number of other cell lines. It has also been used in the study of growth, differentiation, migration of cell lines, and tissue morphogenesis during development.

Rabbit polyclonal anti-Collagen I, N-Terminal antibody reacts with mouse, rat, and human type I collagens.

Imunogênio

The antiserum was produced against synthesized peptide derived from human Collagen I.

Immunogen Range: 1-50

Aplicação

Anti-Collagen I antibody produced in rabbit has been used in western blotting and immunostaining.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Rabbit polyclonal anti-Collagen I, N-Terminal antibody is used to tag collagen type I for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of collagen type I as a cell substrate.

Ações bioquímicas/fisiológicas

Collagen I interacts with cytoskeleton and regulates cell motility. Mutations in this gene is linked to osteoporotic fracture, osteogenesis imperfecta and recessive classical type Ehlers-Danlos syndrome. It is also associated with idiopathic osteoporosis and atypical Marfan syndrome.

Características e benefícios

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forma física

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable