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SAB4200809

Sigma-Aldrich

Anti-Epidermal Growth Factor (EGF) Receptor, Mouse monoclonal

clone 29.1, purified from hybridoma cell culture

Sinônimo(s):

EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

29.1, monoclonal

forma

buffered aqueous solution

peso molecular

~170 kDa

reatividade de espécies

human, bovine, mouse

embalagem

antibody small pack of 25 μL

concentração

~1 mg/mL

técnica(s)

immunoblotting: 0.5-1 μg/mL using human epidermoid carcinoma A431 cell line extract.
immunofluorescence: 2-4 μg/mL using human epidermoid carcinoma A431 cells.

Isotipo

IgG1

nº de adesão UniProt

P00533

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... EGFR(1956)

Descrição geral

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity. EGF binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue.

Imunogênio

Paraformaldehyde-fixed carcinoma cell line A431

Aplicação

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor specifically bind to a carbohydrate residue on the external region of the EGF receptor molecule with no interference to the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to the EGF Receptor. Reactivity has been observed with EGF receptors from mouse, human and bovine origin. The antibody is recommended to use in various immunological techniques, including Immunobloting (~170 kDa), Immunoprecipitation, flow cytometry (FACS), Immunohistochemistry and Immunofluorescence.

forma física

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Outras notas

This product is for R&D use only, not for drug, household, or other uses.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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E Gerdin et al.
American journal of clinical pathology, 99(1), 28-31 (1993-01-01)
It has been reported that endothelium in malignant glioma stains with a commercial antibody raised against the receptor for epidermal growth factor (EGFr) on A431 cells (clone 29.1). In this report, this antibody was used to study the immunohistochemical expression
Y A Kita et al.
FEBS letters, 349(1), 139-143 (1994-07-25)
Her3/erbB3 has been identified as a third member of the epidermal growth factor receptor (EGFR) family [(1989) Proc. Natl. Acad. Sci. USA 86, 9193-9197; (1990) Proc. Natl. Acad. Sci. USA 87, 4905-4909]. The natural ligand for Her3 has not been
L H Defize et al.
The Journal of cell biology, 107(3), 939-949 (1988-09-01)
The epidermal growth factor receptor (EGF-R) of human A431 cells bears an antigenic determinant that is closely related to the human blood group A carbohydrate structure. Labeling studies with blood group A reactive anti-EGF-R monoclonal antibodies and various lectins revealed
Y Yarden et al.
The Journal of biological chemistry, 260(1), 315-319 (1985-01-10)
A method is described for a rapid two-step purification of the membrane receptor for epidermal growth factor (EGF) from cultured human A-431 cells. After solubilization of the cells with Triton X-100, the receptor is immobilized on an immunoaffinity column containing
Alexander Sorkin et al.
Current protocols in cell biology, Chapter 15, Unit 15-Unit 15 (2010-03-18)
Binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) initiates signal transduction, ultimately leading to altered gene expression. Ligand-activated EGFR is also rapidly internalized and then targeted to lysosomes for degradation or recycled back to the plasma membrane.
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