SAB4200597

Monoclonal Anti-FMR1 antibody produced in mouse

clone FMR2.5, hybridoma cell culture supernatant

• Sinônimo(s): FMRP, FRAXA, POF, POF1, fragile X mental retardation 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: FMR2.5, monoclonal
• forma: buffered aqueous solution
• peso molecular: ~71 kDa
• reatividade de espécies: rat, human, mouse, hamster
• concentração: ~1 mg/mL
• técnica(s): flow cytometry: 2.0-5.0 μg/test using HeLa cells; immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts.; immunocytochemistry: suitable; immunofluorescence: 5-10 μg/mL using HepG2 cells.
• Isotipo: IgG1
• nº de adesão UniProt: Q06787
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... FMR1(2332) ; mouse ... Fmr1(14265) ; rat ... Fmr1(24948)

Descrição

Descrição geral

Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm.

Especificidade

Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1.

Imunogênio

synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1

Aplicação

Monoclonal Anti-FMR1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• flow cytometry

Ações bioquímicas/fisiológicas

Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable