SAB4200527

Anti-USP8 antibody, Mouse monoclonal

clone US872, purified from hybridoma cell culture

• Sinônimo(s): Anti-Deubiquitinating enzyme 8, Anti-HumORF8, Anti-UBPY, Anti-Ubiquitin carboxyl-terminal hydrolase 8, Anti-Ubiquitin isopeptidase Y, Anti-Ubiquitin specific peptidase 8, Anti-Ubiquitin thioesterase 8, Anti-Ubiquitin-specific-processing protease 8, Anti-hUBPy, Monoclonal Anti-USP8 antibody produced in mouse
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: US872, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~127 kDa; antigen ~70 kDa
• reatividade de espécies: human, rat, bovine, monkey, mouse, dog
• concentração: ~1.0 mg/mL
• técnica(s): flow cytometry: 5-10 μg/test using MCF7 cells; indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells; western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts
• nº de adesão UniProt: P40818
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... USP8(9101)

Descrição

Descrição geral

Monoclonal Anti-USP8 (mouse IgG1 isotype) is derived from the hybridoma US872 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Ubiquitin specific protease 8 (USP8) belongs to the family of de-ubiquitinating enzymes (DUBs).

Imunogênio

synthetic peptide corresponding to an internal sequence of human USP8, conjugated to KLH.

Aplicação

Monoclonal Anti-USP8 antibody produced in mouse has been used in:immunoblotting immunofluorescence,flow cytometry, immunocytochemistry.

Ações bioquímicas/fisiológicas

Ubiquitin specific protease 8 (USP8) was found to regulate ubiquitination of frizzled, a receptor for Wnt/Wingless. Deletion of USP8 in mice causes embryonic lethality. The defect was accompanied by a strong reduction or absence of several growth factor receptor tyrosine kinases (RTKs), like EGFR, hepatocyte growth factor receptor (c-met), and ERBB3.USP8 inactivation results in enhanced ubiquitination of ligand-activated EGFR. USP8 in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect epidermal growth factor receptor (EGFR) as well as other receptors from lysosomal degradation.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable