SAB4200521

Anti-phospho-VASP [pThr²⁷⁸] antibody produced in rabbit

~0.1 mg/mL, affinity isolated antibody

• Sinônimo(s): Anti-Vasodilator-stimulated phosphoprotein
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~45 kDa
• reatividade de espécies: mouse, human, rat
• concentração: ~0.1 mg/mL
• técnica(s): immunoprecipitation (IP): 40 μL using HEK-293T cells overexpressing human VASP; western blot: 1:5000-10000 using human platelets cell lysates
• nº de adesão UniProt: P50552
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: phosphorylation (pThr278)
• Informações sobre genes: human ... VASP(7408) ; mouse ... Vasp(22323) ; rat ... Vasp(361517)

Descrição

Descrição geral

VASP is localized at highly dynamic membrane regions, focal adhesion sites, lamellipodia protrusions, filopodia tips, and along stress fibers. It is also localized at cell-matrix and cell-cell contacts.

Vasodilator-stimulated phosphoprotein (VASP) is located on human chromosome 19q13.32. It belongs to enabled (Ena) family of proteins and encodes a protein of 46 kDa. VASP has three domain organisation, that is, an N-terminal Ena/VASP (EVH1) homology domain, proline rich central domain and EVH2 actin binding domain.

Especificidade

Anti-phospho-VASP [pThr278] specifically recognizes human VASP phosphorylated at Thr278.

Imunogênio

synthetic peptide containing phosphorylated Thr²⁷⁸ of human VASP, conjugated to KLH. The corresponding sequence is identical in mouse and rat VASP.

Aplicação

Anti-phospho-VASP [pThr278] antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Ações bioquímicas/fisiológicas

Regulation of phosphorylation of Vasodilator-stimulated phosphoprotein (VASP) is observed during actin cytoskeleton formation. It promotes protrusion and propulsion speed and inhibits branching of actins. It plays a key role in the signal transduction pathway related to neutrophil spreading.

VASP plays an important role in cell motility and adhesion. This protein is also involved in adherens junction formation and stabilization in epithelial cells. VASP is a substrate for cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP)-dependent protein kinases. VASP is required for endothelial barrier function in vivo. Knockout of VASP proteins in mice leads to increased endothelial permeability causing fatal vascular leakage and hemorrhage during late embryonic development.

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable