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Key Documents

SAB4200365

Sigma-Aldrich

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-2, purified from hybridoma cell culture

Sinônimo(s):

Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

conjugado

unconjugated

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

PIWIL1-2, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~95 kDa

reatividade de espécies

human, mouse

concentração

~1.0 mg/mL

técnica(s)

immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin® Peroxidase staining system.
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.

Isotipo

IgG2b

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PIWIL1(9271)
mouse ... Piwil1(57749)

Descrição geral

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

Imunogênio

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

Aplicação

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:
  • immunofluorescence
  • immunoprecipitation
  • flow cytometry analysis29}
  • immunoblotting
  • immunohistochemistry

Ações bioquímicas/fisiológicas

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Informações legais

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes
He X, et al.
Nucleic Acids Research, 43(7), 3712-3725 (2015)
High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens
Baumann B, et al.
Prostate, 79(8), 840-855 (2019)
Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas
Qiao D, et al.
Oncogene, 21(25), 3988-3988 (2002)
The biogenesis and function of PIWI proteins and piRNAs: progress and prospect
Thomson T and Lin H
Annual Review of Cell and Developmental Biology, 25, 355-376 (2009)
IL-4 inhibits the biogenesis of an epigenetically suppressive PIWI-interacting RNA to upregulate CD1a molecules on monocytes/dendritic cells
Zhang X, et al.
Journal of Immunology, 196(4), 1591-1603 (2016)

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