SAB4200124

Anti-Nexilin antibody,Mouse monoclonal

clone NX-38, purified from hybridoma cell culture

• Sinônimo(s): Anti-NEXN, Anti-Nelin
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: NX-38, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen 80 kDa
• reatividade de espécies: mouse, human, rat
• embalagem: antibody small pack of 25 μL
• concentração: ~1 mg/mL
• técnica(s): immunocytochemistry: suitable; western blot: 1-2 μg/mL using using rat brain S1 fraction extracts
• Isotipo: IgG2a
• nº de adesão UniProt: Q0ZGT2
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... NEXN(91624) ; mouse ... Nexn(68810) ; rat ... Nexn(246172)

Descrição

Descrição geral

Monoclonal Anti-Nexilin (mouse IgG2a isotype) is derived from the hybridoma NX-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide conjugated to KLH. Nexilin is a filamentous actin-binding protein that localizes to focal contacts. Alternative splicing of the gene yields two different isoforms namely, b-nexilin and s-nexilin.

NEXN (nexilin), a cardiac Z-disc protein is made of 675 amino acids. It is present in the heart and skeletal muscle. NEXN is mapped to human chromosome 1p31.

Especificidade

Monoclonal Anti-Nexilin recognizes human, rat, and mouse nexilin.

Aplicação

Anti-Nexilin antibody,Mouse monoclonal may be used in immunoblotting and immunocytochemistry.

Ações bioquímicas/fisiológicas

NEXN (nexilin) plays an important role in protecting cardiac Z-discs from forces generated within the sarcomere. Mutations in NEXN results in hypertrophic cardiomyopathy.

Nexilin may be involved in cell adhesion and migration.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable