S9396

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

• Sinônimo(s): Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• Número CAS: 9031-54-3
• Número da licença da enzima: 3.1.4.12 (BRENDA, IUBMB)
• Número MDL: MFCD00132362
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• Formulário: buffered aqueous glycerol solution
• Nível de qualidade: 200
• atividade específica: ≥100 units/mg protein (Lowry)
• peso molecular: 34.2 kDa
• atividade externa: Phospholipase C ≤1%
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: Bacillus cereus ATCC 10987 ... spH(2751530)

Descrição

Descrição geral

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

Aplicação

Sphingomyelinase from Bacillus cereus has been used:

• in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
• in lipoprotein modification for the generation of cholesterol crystals in vitro
• to aggregate LDL (low-density lipoprotein)

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.

Ações bioquímicas/fisiológicas

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.

Definição da unidade

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

forma física

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable