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PP0510

Sigma-Aldrich

GlycoProfile™ IV Chemical Deglycosylation Kit

TFMS Deglycosylation System

Sinônimo(s):

Deglycosylation kit

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

Nível de qualidade

200

temperatura de armazenamento

2-8°C

Descrição geral

The GlycoProfile™ IV Kit utilizes trifluoromethane sulfonic acid (TFMS) in a deglycosylation system that completely removes all N- and O-linked glycans while safeguarding the protein or polypeptide structure. TFMS hydrolysis of glycoproteins results in minimal protein degradation while the released glycans are eliminated. The conditions are optimized to deglycosylate simple or low molecular weight glycoproteins in 30 mins without the scavenger. For high molecular weight or complex, non-mammalian glycoproteins, anhydrous anisole is provided as a scavenger to give a high protein yield.

Aplicação

GlycoProfile™ IV Chemical Deglycosylation Kit has been used in the deglycosylation of human α-L-iduronidase and peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera. It has also been used to estimate the glycosylation of plant-derived recombinant human interleukin-13 (rhIL-13).

Características e benefícios

  • Eliminates both N- and O-linked glycans from proteins
  • This enables sequence analysis of the protein core by mass spectrometry (MS) or DNA-to-protein sequence comparison
  • Eliminates glycans which affect the molecular radius
  • Allows molecular mass determination by electrophoresis
  • Complete deglycosylation in as short as 30 mins for high throughput
  • Quick as it eliminates the need for multiple enzyme reactions
  • Accommodates a broad range of glycoproteins
  • Minimal degradation of the protein core to facilitate reliable MS results

Quantidade

The kit contains sufficient reagents for 10 reactions with a sample size between 1-2 mg of a typical glycoprotein. An RNase B glycoprotein standard is included.

Informações legais

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Somente componentes do kit

Nº do produto
Descrição
  • Trifluoromethanesulfonic Acid (TFMS) 5 x 1
  • RNase B Glycoprotein Standard 3 x 1
  • Pyridine solution, 60% 10 mL
  • Bromophenol blue solution, 0.2% .5 mL
  • Anisole, anhydrous 5 x 1
  • Reaction vials 10 ea

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 1 - STOT SE 3

Órgãos-alvo

Eyes,Central nervous system, Respiratory system

Código de classe de armazenamento

3 - Flammable liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

A novel platform for biologically active recombinant human interleukin-13 production
David J W, et al.
Plant Biotechnology (2008)

Artigos

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

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