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Sigma-Aldrich

TruPAGE Precast Gels

10%, 10 x 10cm, 12-well

Sinônimo(s):

Precast Gels for SDS-PAGE, Precast Polyacrylamide Gels

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About This Item

Código UNSPSC:
41105300
NACRES:
NB.22

descrição

for use with Life Technologies, XCell SureLock™ XCell I™, XCell II™ Mini-Cell and Bolt™ Mini Gel Tank

prazo de validade

24 mo. at 2‑8 °C (from the date of manufacture)

embalagem

box of 10 ea

concentração

10% polyacrylamide

cassete L × C

10 cm × 10 cm

espessura do gel

1 mm

tamanho do poço × volume

12 wells × 35 μL

compatibilidade

for use with Lonza Ltd PAGEr Minigel Chamber

temperatura de armazenamento

2-8°C

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Aplicação

TruPAGE Precast Gels is suitable for use for the electrophoretic separation of hepatocytes protein lysate and breast cancer MDA-MB-231 cell lysate prior to western blot analysis.

Características e benefícios

TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The gels come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.

TruPAGE Precast Gels Migration Chart

Informações legais

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
PAGEr is a trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Khedr MEMS, et al.
Cell proliferation, 51(5), e12482-e12482 (2018)
Yousef Risha et al.
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Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can provide valuable information related to breast
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Ferroptosis is a recently discovered form of programmed cell death, but its regulatory mechanisms remain poorly understood. Here, we show that the RNA-binding protein ZFP36/TTP (ZFP36 ring finger protein) plays a crucial role in regulating ferroptosis in hepatic stellate cells

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