P3627
Phosphatase, Acid from wheat germ
≥0.4 unit/mg solid
Sinônimo(s):
(Orthophosphoric-monoester phosphohydrolase [acid optimum] ), Acid Phosphatase from wheat germ
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About This Item
Produtos recomendados
forma
powder
Nível de qualidade
atividade específica
≥0.4 unit/mg solid
peso molecular
58 kDa by gel filtration
temperatura de armazenamento
−20°C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
chave InChI
GZCWLCBFPRFLKL-UHFFFAOYSA-N
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Aplicação
Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.
Ações bioquímicas/fisiológicas
Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.
Qualidade
Contains lipase
Definição da unidade
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.
Nota de preparo
Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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