P302-05

Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• fonte biológica: Porcine pulmonary artery (Normal healthy)
• Nível de qualidade: 100
• Formulário: solid
• embalagem: pkg of 500,000 cells
• fabricante/nome comercial: Cell Applications, Inc
• modo de crescimento: Adherent
• cariótipo: 2n = 38
• morfologia: Endothelial
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): diabetes; cardiovascular diseases
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

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PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

Origem de linhagem celular

Artery

Aplicação

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

Componentes

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Nota de preparo

• 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Rotina de subcultura

Please refer to the PPAEC Culture Protocol.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable