P300-05

Porcine Coronary Artery Endothelial: PCAEC (Cryovial)

• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• fonte biológica: Porcine coronary artery
• Nível de qualidade: 100
• forma: solid
• embalagem: pkg of 500,000 cells
• fabricante/nome comercial: Cell Applications, Inc
• modo de crescimento: Adherent
• cariótipo: 2n = 38
• morfologia: Endothelial
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cardiovascular diseases
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

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Arterial endothelial cells play critical roles in cardiac homeostasis, and insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells provide a useful tool to study artery endothelial function and metabolism.PCAEC from Cell Applications, Inc. have been used to investigate a new mechanism of obesity-associated vascular disease by demonstrating that resistin, an adipocyte-derived cytokine implemented in insulin resistance and diabetes, can cause endothelial dysfunction in coronary arteries through oxidative stress and down-regulation of NO synthase (Kougias, 2005). Conversely, secretoneurin was shown to activate expression of NO synthase and calmodulin, leading to endothelial relaxation (Chan, 2011). PCAEC were also used in adhesion and transmigration experiments in a study investigating anti-inflammatory, cardioprotective effects of a novel adenosine-lidocaine drug formulation (Shi, 2012).

Origem de linhagem celular

Artery

Aplicação

cardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine production

Componentes

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Nota de preparo

• 2nd passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 12 doublings

Rotina de subcultura

Please refer to the PCAEC Culture Protocol.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable