P2922
Endoproteinase Glu-C from Staphylococcus aureus V8
Type XVII-B, lyophilized powder, 500-1,000 units/mg solid
Sinônimo(s):
V8 Protease
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Produtos recomendados
tipo
Type XVII-B
Nível de qualidade
forma
lyophilized powder
atividade específica
500-1,000 units/mg solid
peso molecular
29 kDa
purificado por
chromatography
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044)
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Aplicação
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used to digest reduced and alkylated cyclotides to produce linearized fragments.
It is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.
Ações bioquímicas/fisiológicas
Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.
Definição da unidade
One unit will hydrolyze 1 μmole of N-t-Boc-L-glutamic acid α-phenyl ester per min at pH 7.8 at 37 °C. One unit is equivalent to ~0.004 casein digestion unit.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1 - Skin Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificados de análise (COA)
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Enzymatic Assay of Endoproteinase Glu-C (EC 3.4.21.19)
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