P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• Sinônimo(s): Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• Número CAS: 77107-46-1
• Número MDL: MFCD00131869
• Código UNSPSC: 12352202
• NACRES: NA.61

Propriedades

• fonte biológica: bovine heart
• Nível de qualidade: 200
• Formulário: lyophilized powder
• atividade específica: 2,500-10,000 units/mg protein
• composição: Protein, ≥85% Lowry
• técnica(s): calcium flux assay: suitable
• nº de adesão UniProt: P62157
• temperatura de armazenamento: −20°C
• Informações sobre genes: cow ... CALM3(520277)

Descrição

Aplicação

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Ações bioquímicas/fisiológicas

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Definição da unidade

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

forma física

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Nota de preparo

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)