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Documentos Principais

N7285

Sigma-Aldrich

Ninhydrin Reagent

2% Solution

Sinônimo(s):

1,2,3-Indantrione monohydrate, 2,2-dihydroxyindene-1,3-dione, Ninhydrine

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About This Item

Número CE:
Código UNSPSC:
12352202
NACRES:
NA.56

Aplicação

Ninhydrin reagent has been used to confirm the presence of the amino acid moieties in various reactions.
Ninhydrin reagents can be used in thin layer chromatography (TLC). Ninhydrin reagents have been used to develop and validate a simple, selective, and precise densitometric method for analysis of α-aminocephalosporins, both in bulk drugs and in formulations. Ninhydrin reagents have also been used to observe differences in the reference ranges of several plasma and urine amino acids between Singaporean and Caucasian populations.

Ações bioquímicas/fisiológicas

Ninhydrin is a tricyclic 1,2,3-trione, which functions as an amino acid reagent. It is a vital organic building block, which exposes latent fingerprints on porous surfaces like paper, cardboard and raw wood. Ninhydrin is used to detect primary and secondary amines. It gives a strong blue color with primary amines and a less intense brown-red color with secondary amine.

Outras notas

Ninhydrin and hydrindantin in DMSO and lithium acetate buffer, pH 5.2

Atenção

Store under nitrogen in the refrigerator after use

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Annals of the Academy of Medicine, Singapore, 35(7), 468-475 (2006-08-12)
The analysis of amino acids in plasma and urine was introduced in Singapore when a laboratory for the investigation of inherited metabolic disorders was established by the Ministry of Health. Reference ranges are required for interpreting test results and making
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Gamal A Saleh et al.
Journal of chromatographic science, 48(1), 68-75 (2010-01-09)
A simple, selective, and precise densitometric method for analysis of four alpha-aminocephalosporins, namely cefaclor monohydrate, cefadroxil monohydrate, cefalexin anhydrous, and cefradine anhydrous, both in bulk drugs and in formulations was developed and validated. The method employed thin-layer chromatography (TLC) aluminium
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