N3289

N2-Ethyl-2′-deoxyguanosine

≥98% (HPLC), solid

• Sinônimo(s): N2-Et-dG
• Fórmula empírica (Notação de Hill): C₁₂H₁₇N₅O₄
• Número CAS: 101803-03-6
• Peso molecular: 295.29
• Número MDL: MFCD01631004
• Código UNSPSC: 12352200
• ID de substância PubChem: 329818605
• NACRES: NA.77

Propriedades

• Ensaio: ≥98% (HPLC)
• forma: solid
• cor: white to off-white
• solubilidade: H₂O: >5 mg/mL
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: CCNC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](CO)O3)C(=O)N1
• InChI: 1S/C12H17N5O4/c1-2-13-12-15-10-9(11(20)16-12)14-5-17(10)8-3-6(19)7(4-18)21-8/h5-8,18-19H,2-4H2,1H3,(H2,13,15,16,20)/t6-,7+,8+/m0/s1
• chave InChI: VOKQFDULHQUWAV-XLPZGREQSA-N

Descrição

Descrição geral

N2-Et-dG, an adduct between DNA and the first metabolite of ethanol (acetaldehyde), strongly blocks transcription by RNA polymerases (RNAPs), including mammalian RNAPII and yeast RNAPII, E. coli RNAP, as well as T7 RNAP. All three of the multisubunit RNAPs incorporate a single rNTP residue opposite the N2-Et-dG lesion. The mammalian RNAPII exclusively incorporates cytidine monophosphate (CMP) opposite the N2-Et-dG lesion. The accessory transcription factor TFIIS does not usually act as a lesion bypass factor; instead, it stimulates the polymerase to remove the CMP incorporated opposite the lesion by mammalian RNAPII. N2-Et-dG reduces CTP incorporation by ~1500 fold.

Informações de segurança

• Pictogramas: GHS06,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H300 + H310 + H330,H351,H373
• Declarações de precaução: P202 - P260 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Classificações de perigo: Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Carc. 2 - STOT RE 2
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges