P7181
Polyethylene glycol solution
Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma
Sinônimo(s):
PEG Solution, Polyethylene Glycol
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About This Item
Produtos recomendados
grau
Hybri-Max™
esterilidade
0.2 μm filtered
linha de produto
BioReagent
Formulário
solution
peso molecular
average mol wt 1,450
concentração
50 % (w/v)
técnica(s)
cell culture | hybridoma: suitable
Impurezas
endotoxin, tested
temperatura de armazenamento
2-8°C
cadeia de caracteres SMILES
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
chave InChI
LYCAIKOWRPUZTN-UHFFFAOYSA-N
Categorias relacionadas
Descrição geral
Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.
Aplicação
Polyethylene glycol solution has been used in the hybridization of the following:
- irradiated camel fibroblast cells with A23 hamster cells
- mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
- rabbit splenocytes and cell line 240E-1
Recommended for use in a normal fusion protocol requiring 50% PEG.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Ações bioquímicas/fisiológicas
Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.
Embalagem
Packaged in sealed ampules under nitrogen.
Outras notas
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.
Reconstituição
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.
Informações legais
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
212.0 °F - closed cup
Ponto de fulgor (°C)
100 °C - closed cup
Equipamento de proteção individual
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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