MAK536
Cytotoxicity Assay Kit
sufficient for 100 assays (bioluminescent)
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
Nível de qualidade
aplicação(ões)
pharmaceutical
método de detecção
bioluminescent
doença(s) relevante(s)
cancer
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
Adenosine 5′-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.
Características e benefícios
- As low as 50 cells can be quantified.
- Compatible with high-throughput handling systems.
- No wash and reagent transfer steps are involved.
Adequação
The kit is suitable for:
- Cell proliferation: effects of cytokines, growth factor, nutrients.
- Cytotoxicity and apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.
- Drug discovery: high-throughput screening for anticancer drugs.
Princípio
The Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability, and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3 - Skin Sens. 1
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
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Certificados de análise (COA)
Lot/Batch Number
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