MAK505

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• Nível de qualidade: 100
• aplicação(ões): food and beverages; pharmaceutical
• método de detecção: colorimetric; fluorometric
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damage.

Características e benefícios

• Linear detection range: 6 to 1000 µM for the colorimetric assay and 1 to 100 µM for the fluorometric assay.

Adequação

The kit is suitable for ascorbic acid determination in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture, as well as for studying the effects of drugs on ascorbic acid metabolism.

Princípio

The Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the ascorbic acid concentration in the sample.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H319
• Declarações de precaução: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classificações de perigo: Eye Irrit. 2
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2