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Documentos Principais

MAK443

Sigma-Aldrich

L-Lactate Assay Kit

Sufficient for 100 fluorometric tests

Sinônimo(s):

ʟ-Lactic Acid Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

Nível de qualidade

100

entrada

cell culture medium
plasma
serum
urine

método de detecção

fluorometric

doença(s) relevante(s)

diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer

temperatura de armazenamento

−20°C

Descrição geral

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

Aplicação

The L-Lactate Assay Kit may be used for:
  • Lactate Metabolism Research
  • Tissue Metabolism Research
  • Drug Discovery

Características e benefícios

  • Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
  • Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
  • High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.

Adequação

The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.

Princípio

The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

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