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Merck
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Key Documents

MAK058

Sigma-Aldrich

D-Lactate Colorimetric Assay

sufficient for 100 colorimetric tests

Sinônimo(s):

D-Lactate Test Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

aplicação(ões)

cosmetics
food and beverages

método de detecção

colorimetric

doença(s) relevante(s)

neurological disorders; gastrointestinal diseases

temperatura de armazenamento

−20°C

Descrição geral

D-Lactate, typically present in the bloodstream at nanomolar concentrations, is produced by an intestinal source or via the methylglyoxal pathway. In mammals, D-Lactate metabolism requires D-Lactate hydrogenase and is metabolized slowly, thus an increase in blood concentration levels can lead to acidemia and acidosis. The severity of this D-lactic acidosis can be associated with neurotoxic symptoms. Significant D-Lactate accumulations in the body can also be related to impaired metabolism and excretion.

Aplicação

D-Lactate Colorimetric Assay kit has been used to determine the stereospecificity of lactate produced.

Adequação

Suitable for use with samples of serum, plasma, cells, culture and fermentation media.

Princípio

In this assay, D-Lactate is specifically oxidized by D-Lactate hydrogenase and generates a proportional colorimetric product measured at 450 nm. The useful concentration range in samples is 0.1-10 mM D-Lactate.

Pictogramas

Corrosion

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Dam. 1 - Skin Corr. 1B

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


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Journal of dairy science, 102(11), 9857-9869 (2019-09-16)
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Current eye research, 45(2), 184-189 (2019-08-31)
Purpose: Microbiological investigations of vitreous fluid have often failed to detect the causative agent in infectious endophthalmitis resulting in a clinical dilemma. D-Lactate is a byproduct of bacterial metabolism, and its accumulation in sterile body fluids indicates bacterial infection. The
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The Journal of clinical endocrinology and metabolism, 104(7), 3003-3011 (2019-03-15)
To investigate the link between dietary sugar consumption and two separate pathogenetic mechanisms associated with metabolic syndrome: de novo lipogenesis (DNL) and nonenzymatic glycation. We assessed changes in serum d-lactate (the detoxification end-product of methylglyoxal) concentration in response to 9
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Journal of clinical research in pediatric endocrinology, 12(2), 160-167 (2019-09-14)
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