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Documentos Principais

MAK311

Sigma-Aldrich

Peroxide Assay Kit

sufficient for 250 colorimetric tests

Sinônimo(s):

Hydrogen Peroxide Test Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

método de detecção

colorimetric

doença(s) relevante(s)

cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders

temperatura de armazenamento

2-8°C

Descrição geral

Peroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Aplicação

Peroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium.

Princípio

The Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe3+ −xylenol orange reaction, in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 μM H2O2 equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format.

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Código de classe de armazenamento

8B - Non-combustible corrosive hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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