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Documentos Principais

MAK106

Sigma-Aldrich

CETP Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Sinônimo(s):

Cholesteryl ester transfer protein activity assay kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 fluorometric tests

aplicação(ões)

pharmaceutical

método de detecção

fluorometric

doença(s) relevante(s)

cardiovascular diseases

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... CETP(1071)

Categorias relacionadas

Descrição geral

Cholesteryl ester transfer protein (CETP) is present in normal human plasma. The protein transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway. The method is useful for measuring CETP activity in plasma or serum in all species that express CETP.

Aplicação

Cholesteryl ester transfer protein (CETP) activity Assay Kit has been used for measurement of CETP activity in the plasma samples.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the detection of cholesteryl ester transfer protein (CETP) in plasma / serum (fresh or frozen), recombinant or purified CETP.

Princípio

The CETP Activity Assay Kit uses a proprietary substrate that enables the detection of CETP-mediated transfer of neutral lipid from the substrate to a physiological acceptor. The transfer activity results in an increase in fluorescence intensity (λEx=535 nm/λEm=587 nm). In a total volume of 200 μL, the assay is linear from 0.2 to 0.8 μL of normal human plasma. Assay results not affected by endogenous plasma HDL, LDL or VLDL concentrations.

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Código de classe de armazenamento

10 - Combustible liquids


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Esther M M Ooi et al.
The Journal of clinical endocrinology and metabolism, 96(10), E1568-E1576 (2011-08-06)
Dyslipidemia increases the risk of cardiovascular disease in obesity. Peroxisome proliferator-activated receptor (PPAR)-δ agonists decrease plasma triglycerides and increase high-density lipoprotein (HDL)-cholesterol in humans. The aim of the study was to examine the effect of GW501516, a PPAR-δ agonist, on
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Journal of lipid research, 53(3), 379-389 (2012-01-05)
Mechanisms underlying changes in HDL composition caused by obesity are poorly defined, partly because mice lack expression of cholesteryl ester transfer protein (CETP), which shuttles triglyceride and cholesteryl ester between lipoproteins. Because menopause is associated with weight gain, altered glucose
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Journal of hypertension, 29(5), 863-868 (2011-03-25)
Clinical trials using cholesteryl ester transfer protein (CETP) inhibitors to raise high-density lipoprotein cholesterol (HDL-C) concentrations reported an 'off-target' blood pressure (BP) raising effect. We evaluated the relations of baseline plasma CETP activity and longitudinal BP change. One thousand, three
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[Figure: see text].
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American journal of respiratory and critical care medicine, 199(7), 854-862 (2018-10-16)
High-density lipoprotein (HDL) cholesterol (HDL-C) levels decline during sepsis, and lower levels are associated with worse survival. However, the genetic mechanisms underlying changes in HDL-C during sepsis, and whether the relationship with survival is causative, are largely unknown. We hypothesized

Protocolos

CETP Activity Assay Kit Protocol

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