MAK122
Phospholipid Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Sinônimo(s):
Phospholipid Quantification Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
uso
sufficient for 100 colorimetric or fluorometric tests
aplicação(ões)
cosmetics
food and beverages
pharmaceutical
método de detecção
colorimetric
fluorometric
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
Aplicação
Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.
Características e benefícios
Compatible with high-throughput handling systems.
Adequação
Suitable for the detection of phospholipids in biological samples
Princípio
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3 - Resp. Sens. 1
Código de classe de armazenamento
10 - Combustible liquids
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