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MAK025

Sigma-Aldrich

Iron Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

aplicação(ões)

cosmetics
food and beverages

método de detecção

colorimetric

doença(s) relevante(s)

cancer; hematological disorder

temperatura de armazenamento

−20°C

Descrição geral

Iron is a mineral that plays an essential role in many biological processes, including DNA synthesis, electron transport and oxygen transport. Iron is a transition element that can form a range of oxidation states, the most common being iron II (Fe2+ or ferrous iron) and iron III (Fe3+ or ferric iron). Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions.

Aplicação

Iron assay kit has been used to determine the concentration of Fe2+ in wild type (WT) and Δ ferric uptake regulator (Fur) strains of A. baumannii.

Adequação

Suitable for the measurement of iron concentration in various samples

Princípio

In this assay, iron is released by the addition of an acidic buffer. Samples may be tested directly to measure Fe2+ or reduced to measure total iron (Fe2+ and Fe3+). Released iron is reacted with a chromagen resulting in a colorimetric (593 nm) product, proportional to the iron present. The Iron Assay Kit provides a simple convenient means of measuring iron in a variety of biological samples.

Nota de preparo

Kit is compatible with serum and tissue samples but not plasma samples.

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Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


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