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Key Documents

RAB0197

Sigma-Aldrich

Human Ferritin ELISA Kit

for serum, plasma, cell culture supernatant and urine

Sinônimo(s):

FTH, Ferritin H subunit, Ferritin heavy chain

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

reatividade de espécies

human

embalagem

kit of 96 wells (12 strips x 8 wells)

técnica(s)

ELISA: suitable
capture ELISA: suitable

entrada

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.81-200 ng/mL

método de detecção

colorimetric

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... FTH1(2495)

Descrição geral

The Human Ferritin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ferritin in serum, plasma, cell culture supernatants and urine.
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).

Imunogênio

Recombinant Human Ferritin

Aplicação

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Ferritin ELISA Kit has been used to estimate the amount of ferritin in cellular suspension.
Human ferritin ELISA kit. has been used to measure the ferritin secreted in supernatants from THP-1 (tamm-horsfall protein 1) cells that were sequentially treated with lipopolysaccharides (LPS) and LLOMe (a lysosome disruption agent).

Ações bioquímicas/fisiológicas

Ferritin mediates iron metabolism and plays a key role in redox homeostasis. Ferritin possesses ferroxidase activity and maintains iron in its non-toxic form. Ferritin acts as an iron-storage protein and stores excessive intracellular iron that is not utilized for the production of heme. Also, iron is released from ferritin upon degradation.
Ferritin/ ferritin heavy chain 1 (FTH1) is a cytoplasmic protein. It acts as a cargo for transport to autophagosomes for degradation. FTH1 maintains the cellular redox status by storing the intracellular iron in a non-toxic and readily available form. It acts as a key antioxidant molecule in various physiologic and pathologic conditions, such as apoptosis, inflammation, vascular protection and neurodegeneration. FTH1 alone or along with ferritin light chain is implicated to detect cancer cells, stem cells, fibroblasts and dendritic cells.

Outras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Met. Corr. 1

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Epithelial-to-mesenchymal transition in FHC-silenced cells: the role of CXCR4/CXCL12 axis.
Aversa I, et al.
Journal of Experimental & Clinical Cancer Research, 36(1), 104-104 (2017)
In Vivo magnetic resonance imaging of xenografted tumors using FTH1 reporter gene expression controlled by a tet-on switch
He X, et al.
Oncotarget, 7(48), 78591-78591 (2016)
shRNA targeting of ferritin heavy chain activates H19/miR-675 axis in K562 cells.
Di Sanzo M, et al.
Gene, 657, 92-99 (2018)
Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy.
Kimura T, et al.
The Embo Journal, e201695081-e201695081 (2016)
Nishant Kumar Ojha et al.
Molecular and cellular biochemistry, 417(1-2), 75-85 (2016-05-14)
Hepatitis E Virus (HEV) is the major causative agent of acute hepatitis in developing countries. Its genome has three open reading frames (ORFs)-called as ORF1, ORF2, and ORF3. ORF1 encodes nonstructural polyprotein having multiple domains, namely: Methyltransferase, Y domain, Protease

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