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L9420

Sigma-Aldrich

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Sinônimo(s):

Luciferase firefly

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About This Item

Número CAS:
61970-00-1
Número da licença da enzima:
1.13.12.7 (BRENDA, IUBMB)
Número MDL:
MFCD00131546
Código UNSPSC:
12352204
NACRES:
NA.54

recombinante

expressed in E. coli

Nível de qualidade

200

Ensaio

≥98% (SDS-PAGE)

forma

buffered aqueous solution

atividade específica

≥10×1010 units/mg protein

peso molecular

62 kDa

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Aplicação

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Definição da unidade

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

forma física

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H302,H334,H373

Classificações de perigo

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

Órgãos-alvo

Kidney

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

231.8 °F

Ponto de fulgor (°C)

111 °C

Certificados de análise (COA)

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Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the Karrikin signaling pathway.
Sun Y K, et al.
Frontiers in Plant Science, 7, 1799-1799 (2016)
Greta Forlani et al.
Methods in molecular biology (Clifton, N.J.), 1087, 299-313 (2013-10-26)
Human T cell Lymphotropic Viruses 1 and 2 (HTLV-1 and HTLV-2) are the first described human retroviruses. HTLV-1 is the causative agent of an aggressive malignancy of CD4+ T lymphocytes named adult T-cell leukemia/lymphoma (ATLL) and of a chronic neurological
Junji Chida et al.
Methods in molecular biology (Clifton, N.J.), 1098, 21-32 (2013-10-30)
Adenosine 5'-triphosphate (ATP) is the "energy currency" of organisms and plays central roles in bioenergetics, whereby its level is used to evaluate cell viability, proliferation, death, and energy transmission. In this chapter, we describe an improved and efficient method for
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